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rabbit anti mmp 2 pab  (Bioss)


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    Bioss rabbit anti mmp 2 pab
    Rabbit Anti Mmp 2 Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mmp+2+pab/MMP2+Polyclonal+Antibody/pmc12783216-68-18-24
    Average 94 stars, based on 63 article reviews
    rabbit anti mmp 2 pab - by Bioz Stars, 2026-10
    94/100 stars

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    Western Blot:

    Article Title: BMSC-Derived Exosomal miR-21a-5p Ameliorates Blood-Brain Barrier Injury and Hemorrhagic Transformation.
    Article Snippet: .. Western blotting antibodies were: rabbit anti-Claudin-5 pAb (PA5-99,415; RRID: AB_2818348; Invitrogen), rabbit anti-ZO-1 pAb (ab216880; RRID: AB_2909434; Abcam), rabbit anti-MMP-2 pAb (bs0412R; RRID: AB_10856777; Bioss, China), rabbit antiOccludin mAb (ab216327; RRID: AB_2737295; Abcam), rabbit recombinant anti-MMP- 9 (ab283575; RRID: AB_2928971; Abcam), rabbit anti-TLR2 mAb (ab209216; RRID: AB_3662636; Abcam), rabbit recombinant anti-pP65 (Ser468) (82,335–1-RR; RRID: AB_3083091; Proteintech, USA), rabbit recombinant anti-P65 (80,979–1-RR; RRID: AB_2918923; Proteintech) and mouse anti-β-actin mAb (ab8226; RRID: AB_306371; Abcam). ..

    Article Title: BMSC-Derived Exosomal miR-21a-5p Ameliorates Blood–Brain Barrier Injury and Hemorrhagic Transformation
    Article Snippet: .. Western blotting antibodies were: rabbit anti-Claudin-5 pAb (PA5-99,415; RRID: AB_2818348; Invitrogen), rabbit anti-ZO-1 pAb (ab216880; RRID: AB_2909434; Abcam), rabbit anti-MMP-2 pAb (bs-0412R; RRID: AB_10856777; Bioss, China), rabbit anti-Occludin mAb (ab216327; RRID: AB_2737295; Abcam), rabbit recombinant anti-MMP- 9 (ab283575; RRID: AB_2928971; Abcam), rabbit anti-TLR2 mAb (ab209216; RRID: AB_3662636; Abcam), rabbit recombinant anti-p-P65 (Ser468) (82,335–1-RR; RRID: AB_3083091; Proteintech, USA), rabbit recombinant anti-P65 (80,979–1-RR; RRID: AB_2918923; Proteintech) and mouse anti-β-actin mAb (ab8226; RRID: AB_306371; Abcam). ..

    Recombinant:

    Article Title: BMSC-Derived Exosomal miR-21a-5p Ameliorates Blood-Brain Barrier Injury and Hemorrhagic Transformation.
    Article Snippet: .. Western blotting antibodies were: rabbit anti-Claudin-5 pAb (PA5-99,415; RRID: AB_2818348; Invitrogen), rabbit anti-ZO-1 pAb (ab216880; RRID: AB_2909434; Abcam), rabbit anti-MMP-2 pAb (bs0412R; RRID: AB_10856777; Bioss, China), rabbit antiOccludin mAb (ab216327; RRID: AB_2737295; Abcam), rabbit recombinant anti-MMP- 9 (ab283575; RRID: AB_2928971; Abcam), rabbit anti-TLR2 mAb (ab209216; RRID: AB_3662636; Abcam), rabbit recombinant anti-pP65 (Ser468) (82,335–1-RR; RRID: AB_3083091; Proteintech, USA), rabbit recombinant anti-P65 (80,979–1-RR; RRID: AB_2918923; Proteintech) and mouse anti-β-actin mAb (ab8226; RRID: AB_306371; Abcam). ..

    Article Title: BMSC-Derived Exosomal miR-21a-5p Ameliorates Blood–Brain Barrier Injury and Hemorrhagic Transformation
    Article Snippet: .. Western blotting antibodies were: rabbit anti-Claudin-5 pAb (PA5-99,415; RRID: AB_2818348; Invitrogen), rabbit anti-ZO-1 pAb (ab216880; RRID: AB_2909434; Abcam), rabbit anti-MMP-2 pAb (bs-0412R; RRID: AB_10856777; Bioss, China), rabbit anti-Occludin mAb (ab216327; RRID: AB_2737295; Abcam), rabbit recombinant anti-MMP- 9 (ab283575; RRID: AB_2928971; Abcam), rabbit anti-TLR2 mAb (ab209216; RRID: AB_3662636; Abcam), rabbit recombinant anti-p-P65 (Ser468) (82,335–1-RR; RRID: AB_3083091; Proteintech, USA), rabbit recombinant anti-P65 (80,979–1-RR; RRID: AB_2918923; Proteintech) and mouse anti-β-actin mAb (ab8226; RRID: AB_306371; Abcam). ..



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    rabbit anti mmp 2 pab  (Bioss)
    94
    Bioss rabbit anti mmp 2 pab
    Rabbit Anti Mmp 2 Pab, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mmp+2+pab/MMP2+Polyclonal+Antibody/pmc12783216-68-18-24
    Average 94 stars, based on 1 article reviews
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    mmp 2  (Sino Biological)
    93
    Sino Biological mmp 2
    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
    Mmp 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mmp 2  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology mmp 2
    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
    Mmp 2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti mmp 2  (ABclonal Biotechnology)
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    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
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    mmp 2 a6247  (ABclonal Biotechnology)
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    ABclonal Biotechnology mmp 2 a6247
    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
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    mmp 2 a6247 antibodies  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology mmp 2 a6247 antibodies
    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
    Mmp 2 A6247 Antibodies, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse mmp 2  (Sino Biological)
    91
    Sino Biological mouse mmp 2
    A: Western blotting was used to detect the expression of <t>MMP-2</t> and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.
    Mouse Mmp 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A: Western blotting was used to detect the expression of MMP-2 and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

    Journal: Journal of Traditional Chinese Medicine

    Article Title: Saponin Ⅰ from Shuitianqi ( Rhizoma Schizocapasae Plantagineae ) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg’s Albino/c mouse model

    doi: 10.19852/j.cnki.jtcm.2024.04.006

    Figure Lengend Snippet: A: Western blotting was used to detect the expression of MMP-2 and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

    Article Snippet: Subsequently, sections were incubated in 5% BSA blocking fluid for 1 h, with subsequent ON incubation in primary antibodies at 4 ℃: E-cadherin, N-cadherin, Vimentin, TGF-β1(PTG, 21898-1-AP), Smad7, MMP-2, MMP-9, CD31, CD34 (Sino Biological Inc., 50589-T24), and VEGF (all dilution 1:200).

    Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Saline, Injection, Standard Deviation