rabbit anti mmp 2 pab  (Bioss)

Journal: Journal of Traditional Chinese Medicine

Article Title: Saponin Ⅰ from Shuitianqi ( Rhizoma Schizocapasae Plantagineae ) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg’s Albino/c mouse model

doi: 10.19852/j.cnki.jtcm.2024.04.006

Figure Lengend Snippet: A: Western blotting was used to detect the expression of MMP-2 and MMP-9 proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of MMP-2 and MMP-9 proteins expression. C: immunohistochemical assay was used to detect the expression of MMP-2 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of MMP-9 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: quantitative analysis of MMP-2 and MMP-9 proteins expression. F: enzyme linked immunosorbent assay was used to detect the expression of MMP-2 protein in serum. G: enzyme linked immunosorbent assay was used to detect the expression of MMP-9 protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); MMP: matrix etalloproteinase; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

Article Snippet: Subsequently, sections were incubated in 5% BSA blocking fluid for 1 h, with subsequent ON incubation in primary antibodies at 4 ℃: E-cadherin, N-cadherin, Vimentin, TGF-β1(PTG, 21898-1-AP), Smad7, MMP-2, MMP-9, CD31, CD34 (Sino Biological Inc., 50589-T24), and VEGF (all dilution 1:200).

Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Saline, Injection, Standard Deviation

Journal: Cancers

Article Title: Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness

doi: 10.3390/cancers14235917

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: pAb Rabbit anti-human MMP-9 (#10375-2-AP) , // , 1:500 , // , Proteintech Group, Manchester, UK.

Techniques: